PROTOPLAST FUSION
NAMA : ARYA WIRA WARDHANA NPM : 21025010035 KELAS : A025 MATA KULIAH : BIOTEKNOLOGI Protoplast Fusion Enzymatic isolation is advantageous because protoplasts are gained at a high quantity, cells are not damaged and the osmotic conditions may be influenced. Enzymatic isolation may be carried out in two different procedures: two-step or one-step procedure. In the first step of the two-step procedure, individual cells are released with the help of commercial enzymatic preparations (e.g. macerozyme, macerase). The cells are released by degradation of the middle lamellas and the decay of tissue to individual cells. The free cells are then processed in the second step with the help of cellulases (cellulase Onozuka R-10, cellulysin) to protoplasts by dissolving the cell wall. The cells are exposed to the enzymes for a shorter time than at one-step isolation. One-step isolation is used more often, during which the mechanically loosened tissue (e.g. by cutting strips) is ...